Effects of salinity on anaerobic digestion: Performance, microbial physiology, and community dynamics

Anaerobic digestion (AD) is widely applied to treatment and energy recovery from organic wastewater/wastes, while the efficiency of AD can be limited by salinity stress. This paper reviews the effects of salinity on AD. First of all, the effects of salinity on AD performance were compared, revealing that methane production is more susceptible to salinity stress. Secondly, the influence of salinity on microbial physiology and intracellular molecules was examined, demonstrating that salinity stress reduces the activity of key enzymes and increases the concentration of extracellular polymeric substances during AD. Thirdly, variations in microbial community structure under salinity stress were discussed, with archaeal communities showing more significant restructuring, including reduced dominance of acetoclastic methanogens. At last, strategies to mitigate salinity inhibition were presented, along with prospects for future research directions. This review provides theoretical guidance for engineering applications and strategies for enhancing AD in treating saline substrates.