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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...

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Related Experiment Video

Updated: May 12, 2025

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Heterohybridomas producing human immunoglobulin light chains using CD138-selected bone marrow cells.

P Zhou¹, X Ma¹, S Scalia¹

¹Tufts Medicine Myeloma and Amyloid Program, USA.

Biochemistry and Biophysics Reports

|May 7, 2025

Summary

Heterohybridoma (HH) technology successfully produced human light chain (FLC) producing clones from multiple myeloma and polyclonal gammopathy patients. These FLC-producing HH clones offer valuable tools for studying FLC behavior and developing new therapeutics.

Keywords:

CD138 Heterohybridomas Immunoglobulin light chains Plasma cells

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Last Updated: May 12, 2025

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

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Published on: February 6, 2018

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

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Published on: June 5, 2015

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Area of Science:

Immunology
Biotechnology

Background:

Mammalian cell lines for human light chain (FLC) production are scarce, hindering research.
Heterohybridoma (HH) technology offers a novel approach to generate these essential tools.

Purpose of the Study:

To develop and characterize heterohybridoma (HH) clones capable of producing human light chains (FLC).
To provide valuable tools for studying FLC behavior, metabolism, and therapeutic targeting.

Main Methods:

Selected CD138+ cells from multiple myeloma (MM) and polyclonal gammopathy (PG) patients.
Fused selected cells with B5-6 T cells and cultured using hypoxanthine-aminopterin-thymidine (HAT) medium.
Selected and characterized HH clones using ELISA, flow cytometry, and RT-PCR for FLC production, intracellular levels, and gene sequencing.

Main Results:

Successfully generated FLC-producing HH clones from 50% of MM and PG cases.
HH clones demonstrated robust intracellular FLC production (median 9849 MFI) and secretion (median 47 μg/mL in vitro, 66.4 μg/mL in vivo).
Observed dimer/monomer ratios in vitro and in vivo, with increased dimer formation in vivo.

Conclusions:

Heterohybridoma (HH) technology is effective for producing human light chain (FLC) secreting clones.
Generated HH clones provide a platform for studying FLC metabolism and evaluating FLC-targeting therapeutics.
Further research can utilize these HH clones to investigate FLC-related diseases and develop novel treatment strategies.