Determination of polysorbate in ascites fluid from a premature infant

Area of Science:

• Analytical Chemistry
• Biochemistry
• Clinical Chemistry

Background:

• Poly(oxy-1,2-ethanediyl) oligomers, often used as surfactants, can be present in pharmaceutical formulations.
• Monitoring these compounds in biological samples is crucial for assessing patient safety, especially in vulnerable populations like neonates.
• Existing analytical methods may lack the sensitivity or specificity required for accurate quantification in complex matrices like body fluids.

Purpose of the Study:

• To develop and validate a sensitive method for determining poly(oxy-1,2-ethanediyl) oligomers in human body fluids.
• To investigate the presence and levels of these oligomers in peritoneal fluid of infants receiving vitamin E supplements.

Main Methods:

• Utilized ammonium cobaltothiocyanate complexation for selective binding of the target analytes.
• Employed high-pressure liquid chromatography (HPLC) for efficient separation of the oligomers.
• Applied visible spectrophotometry for sensitive and quantitative detection of the complexed oligomers.

Main Results:

• Successfully established a method for the determination of poly(oxy-1,2-ethanediyl) oligomers.
• Analysis of peritoneal fluid samples from an infant treated with E-ferol (vitamin E supplement) revealed significant levels of polysorbates.
• Detected concentrations reached up to 100 micrograms/mL, indicating substantial exposure.

Conclusions:

• The developed analytical method is effective for quantifying poly(oxy-1,2-ethanediyl) oligomers in body fluids.
• The findings highlight the potential for high systemic levels of these compounds following administration of certain vitamin E formulations.
• Further research is warranted to understand the clinical implications and long-term effects of such exposure in infants.