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Updated: May 12, 2025
A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch
Published on: December 10, 2020
Macrophage membrane functionalized composite microspheres promote bone regeneration in periodontitis via manipulating
Rui Song1, Zhuo Wan2, Xiaojing Yuan1
1Department of Pediatrics, Peking University School and Hospital of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health, NMPA Key Laboratory for Dental Materials, Beijing, 100081, PR China.
Abstract:
Periodontitis is characterized by inflammation and alveolar bone loss, primarily caused by immune cells activated by oral bacteria, leading to an imbalance between osteogenesis and bone resorption. Traditional treatments have limited efficacy, which has led to the exploration of regulating the immune microenvironment and utilizing tissue engineering methods as new research directions. Our study demonstrates that macrophage membranes, activated by LPS and IFN-γ, can effectively neutralize inflammatory factors. By coating the poly-L-lysine (PLL) modified poly (lactic-co-glycolic acid) (PLGA)/β-TCP microspheres with such macrophage membrane vesicles, the MM@PPT microspheres regulate intercellular responses by inhibiting macrophage M1 polarization and osteoclast differentiation, promoting M2 polarization, and enhancing osteogenic differentiation of bone marrow stromal cells (BMSCs) even in an inflammatory environment. By injecting the MM@PPT into sites of periodontitis induced bone resorption, it is found that they can effectively promote bone regeneration by modulating the immune-regeneration microenvironment. This work not only highlights the potential of MM@PPT microspheres in promoting alveolar bone regeneration but also provides insights into how these microspheres modulate cell behavior and interactions. The findings of this study offer novel therapeutic strategies for promoting alveolar bone repair in periodontitis.
