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Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series
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Revealing Rotational Characteristics of the Uniflagellate Mutant of Chlamydomonas reinhardtii through
Azusa Kage1, Ken H Nagai2, Takayuki Nishizaka3
1Graduate School of Engineering, Muroran Institute of Technology, Muroran, Hokkaido, Japan.
Abstract:
Tracking eukaryotic flagella and cilia often requires manual clicking, even in the age of digital imaging. We developed an autotracking method using DeepLabCut, a CNN-based, marker-less tracking tool originally designed for animal behavior. Applying this method, we uncovered rotational characteristics of Chlamydomonas reinhardtii uni1 , a uniflagellate mutant. Live uni1 cells predominantly rotated counterclockwise under a coverslip when viewed from above, whereas demembranated models exhibited slower, more clockwise rotation. These differences likely stem from alterations in the three-dimensional flagellar waveform.
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