RRMScorer: A web server for predicting RNA recognition motif binding preferences

RRMScorer is a web server designed to predict RNA binding preferences for proteins containing RNA recognition motifs (RRMs), the most prevalent RNA binding domain in eukaryotes. By carefully analysing a dataset of 187 RRM-RNA structural complexes, we calculated residue-level binding scores using a probabilistic model derived from amino acid-nucleotide interaction propensities, which are the basis of RRMScorer. The server accepts protein sequences and optional RNA sequences as input, providing detailed outputs, including bar plots, sequence logos, and downloadable CSV/JSON files, to visualize and interpret RNA binding preferences. RRMScorer is particularly useful for studying the impact of single-point mutations and comparing binding preferences across multiple RRM domains. The web server, freely accessible at https://bio2byte.be/rrmscorer without login requirements, offers a user-friendly interface and integrates precomputed predictions for over 1400 RRM-containing proteins. With its ability to provide residue-level insights and accurate predictions, RRMScorer serves as a valuable tool for researchers exploring the functional landscape of RRM-RNA interactions.