[Typical strategy and research progress of efficient isolation methods of exosomes based on affinity interaction]

Area of Science:

• Biochemistry
• Cell Biology
• Nanotechnology

Background:

• Exosomes, a subclass of extracellular vesicles, are secreted by most cells and found in bodily fluids.
• They act as critical intercellular communication mediators.
• Exosomes are vital for disease diagnosis, prognosis, and treatment, functioning as important liquid-biopsy tools.

Purpose of the Study:

• To review recent advancements in exosome isolation and enrichment techniques.
• To focus on efficient isolation methods based on affinity interactions.
• To analyze future prospects for exosome isolation strategies.

Main Methods:

• Systematic review of scientific literature on exosome isolation.
• Focus on affinity-interaction-based isolation methods.
• Analysis of traditional physical, chemical, and biological isolation techniques.

Main Results:

• Efficient isolation of high-quality exosomes is challenging due to low abundance and interference from other macromolecules.
• Affinity-interaction-based methods have overcome limitations of traditional isolation techniques.
• These advanced methods are increasingly used in research and clinical applications.

Conclusions:

• Affinity-based exosome isolation represents a significant advancement.
• Improved isolation techniques are essential for accurate disease diagnosis and treatment.
• Further development in exosome isolation strategies is needed for clinical utility.