Related Experiment Video
Updated: Jun 12, 2025

Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
Antineutrophil Cytoplasmic Antibodies (ANCAs): Role in Diagnosis, Disease Monitoring, and Prognosis
Nicole E Wyatt1, Vimal K Derebail1, Ronald J Falk1,2
1Division of Nephrology and Hypertension, Department of Medicine, UNC Kidney Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
Abstract:
ANCA-associated vasculitis (AAV) is a rare and potentially fatal autoimmune disorder characterized by pauci-immune necrotizing vasculitis affecting small- to medium-sized blood vessels. The pathogenic role of ANCAs in AAV is supported by both clinical and experimental evidence, and when used in the proper clinical setting, ANCA testing is highly specific for AAV. Testing with both indirect immunofluorescence assay and enzyme immunoassay may increase sensitivity for AAV; however, testing with a high-quality enzyme immunoassay may be used alone. Nonvasculitic conditions and drugs can cause ANCA positivity without manifestations of AAV. We review ANCA testing itself, performance characteristics and specific conditions for the laboratory test, and various conditions when ANCA testing is useful in diagnosis, disease monitoring, and selecting treatment.
More Related Videos
07:52Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
Published on: September 10, 2018
11:32Real-Time, High-Throughput Microscopic Quantification of Human Neutrophil Extracellular Trap Release and Assessing the Pharmacology of Antagonists
Published on: October 18, 2024
Related Concept Videos
Acute Pyelonephritis II: Diagnostic Studies and Management
Nephrotic Syndrome II : Assessment and Medical Management
Acute Kidney Injury IV: Diagnostic Studies and Prevention
Pneumonia III: Complications and Assessment
Acute Coronary Syndrome III: Diagnostic studies
Nephrotic Syndrome III : Nursing Management