Association between perfluorooctanoic acid-related poor embryo quality and metabolite alterations in human follicular fluid during IVF：A Cohort Study
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View abstract on PubMed
Summary
This summary is machine-generated.High levels of perfluoroalkyl and polyfluoroalkyl substances (PFAS) in follicular fluid negatively impact embryo quality during assisted reproductive technology (ART). Perfluorooctanoic acid (PFOA) is a major contributor, linked to reduced essential metabolites and poorer pregnancy outcomes.
Area Of Science
- Environmental Health Sciences
- Reproductive Toxicology
- Metabolomics
Background
- Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are known to disrupt reproductive functions.
- Specific PFAS in follicular fluid impacting oocyte and embryo development remain unidentified.
- The role of follicular fluid metabolites in PFAS-induced adverse effects is unclear.
Purpose Of The Study
- To identify specific PFAS in follicular fluid that adversely affect embryo quality.
- To investigate the modes of action for identified PFAS.
- To explore the contribution of follicular fluid metabolites to these effects.
Main Methods
- Cross-sectional and cohort study involving 378 women undergoing assisted reproductive technology (ART).
- PFAS detection and untargeted metabolomics on follicular fluid samples.
- Statistical analyses including beta regression, logistic regression, Bayesian kernel machine regression, and causal mediation effect models.
Main Results
- Fifteen PFAS were detected in over 85% of samples, with perfluorooctanoic acid (PFOA) showing the highest median concentration.
- PFAS mixtures were negatively associated with high-quality embryo rates, with PFOA as the primary contributor.
- Embryo quality mediated the relationship between PFOA, perfluoro-n-nonanoic acid (PFNA), and perfluoro-n-tridecanoic acid (PFTrDA) and clinical pregnancy outcomes.
Conclusions
- Elevated follicular fluid PFAS levels correlate with reduced embryo quality in ART.
- PFOA is a significant contributor to poor embryo quality, potentially by decreasing essential organonitrogen and sphingolipid metabolites.
- These metabolite changes impact normal cell growth and metabolism, affecting reproductive success.
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