Light-regulated dentification and dissimilatory nitrate reduction by nano-bio electric syntrophic consortium

Microbial-oriented nitrogen recycling is a vital strategy for nitrogen pollution control in the treatment of low C/N wastewater. However, the deficient electron donors in water body limits the reactive nitrogen recovery. Herein, we design a nano-bio electric syntrophic consortium for light-regulated dentification and dissimilatory nitrate reduction to ammonium (DNRA) without organic carbon sources input. Using Fe⁰ coupons as the sole electron donor, the extracellular electron uptake rate of a model denitrifier (Pseudomonas aeruginosa PAO1) is enhanced by coculturing it with an electroactive bioregulator, Shewanella oneidensis MR-1 (MR-1), thereby achieving an average nitrate removal rate of 63.8 ± 0.1 mg N/d/L with ammonium recovery efficiency of 27.1 ± 0.2 % under illumination. Notably, in situ self-assembled FeS nanoparticles via a bottom-up Fe⁰ biocorrosion approach are observed on the outer membrane and periplasmic space of MR-1. Under illumination, native MtrCOmcA-CymA protein complex and FeS nanoparticles act in electron conduits to facilitate transmembrane photoelectron uptake of MR-1 for microbial DNRA process. Biochemical and transcriptomic analyses reveal that the NADH generation, chemotaxis moving and energy-taxis of MR-1 hybrids strength the driving force for microbial DNRA process. Overall, we demonstrate that the constructed FeS-assisted coculture, as an emerging model of electric syntrophy, could support the solar-triggered nitrogen metabolism from non-phototrophic microbes. Given that Fe⁰ biocorrosion is a facile route to MR-1 growth, this nano-bio system also affords a promising pathway for low C/N wastewater treatment and reactive nitrogen recovery via DNRA process.