Cascade effects of nutrient input on river microeukaryotic stability: habitat heterogeneity-driven assembly...

The assembly process and stability mechanism of microeukaryotes can reflect the health and sustainability of river ecosystems, and changes in land use types can alter biodiversity and affect ecosystem functions. Here, we used 18S rDNA amplicon sequencing technology to explore the effects of land use and dry and wet season changes on microeukaryotic species composition, community assembly, co-occurrence networks, and network stability, as well as the mechanisms driving observed changes. The total phosphorus concentration was 13.3 and 7.8 times higher and the total nitrogen concentration was 6.3 and 3.8 times higher in agricultural and urban river sections, respectively, than in forest river sections. Differences in land use types have created heterogeneity on river habitats and altered the distribution and species composition of microeukaryotes, reducing the number and diversity of endemic species in communities and simplifying the food web. High nitrogen and phosphorus inputs promoted the abundance of low-trophic-level species; ecosystem stability and population sizes were maintained by high trophic levels, which controlled the abundance of low trophic levels through predation and promoted nitrogen transformation. The high-nutrient environment reduced the niche breadth of species (>70 % dry season niche breadth contraction), thus promoting specialization; given that this placed these species at a disadvantage in the competition for resources, community stability decreased (60 %/40 % wet/dry season robustness reductions). The physical dilution effect of the river in the dry season was weakened, and the input of domestic sewage and agricultural return water promoted deterministic processes (71.43 % increased |βNTI|>2 in dry season). The environmental filtration effect in the wet season was still stronger than the physical dilution effect caused by the increase in river flow (neutral model R² = 33.5 %). The input of large amounts of nutrients was the main driver of the decline in the stability of microeukaryotes (Total Effect = -0.62).