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Updated: Jul 14, 2026

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
Published on: October 5, 2016
VvNAC33 functions as a key regulator of drought tolerance in grapevine by modulating reactive oxygen species
Na Xu1, Songlin Zhang2, Xiaoming Zhou2
1College of Life Science and Technology, Xinjiang University, Urumqi, China; The State Key Laboratory of Genetic Improvement and Germplasm Innovation of Crop Resistance in Arid Desert Regions (Preparation), Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables, Institute of Fruits and Vegetables, Xinjiang Academy of Agricultural Sciences, Urumqi, China.
Abstract:
Grapevine (Vitis vinifera L. and other Vitis spp.) is an important economic crop, but its yield and quality are severely affected by drought stress. NAC transcription factors, which play key roles in plant stress responses, have remained largely unexplored in grapevine drought tolerance. This study identified VvNAC33 as a drought-responsive candidate gene through transcriptomic analysis and demonstrated its role as a positive regulator of drought tolerance. VvNAC33 expression was significantly upregulated under drought stress. Subcellular localization and transcriptional activity analyses confirmed its nuclear localization and transcriptional activation potential. Overexpression of VvNAC33 in Arabidopsis thaliana and transient overexpression in grapevine enhanced drought tolerance, whereas virus-induced gene silencing increased drought sensitivity. This enhanced tolerance was associated with the activation of the antioxidant defense system, including superoxide dismutase, peroxidase, and catalase, which promoted reactive oxygen species scavenging and alleviated oxidative damage. The enhanced expression of VvCAT1, VvCu/ZnSOD, and VvPOD4 by VvNAC33 highlights its crucial role in regulating antioxidant gene expression under drought stress. These findings strongly support the role of VvNAC33 in drought tolerance and identify it as a potential molecular target for enhancing drought resistance in grapevine.
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