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Published on: August 8, 2019
Evolution of Chlorella sp. RCC288 proteome and transcriptome during its adaptation to oil mill wastewater
Bouthaina Menaa1, Rihab Hachicha1, Pascal Dubessay2
1Laboratory of Plant Biotechnologies Applied to Crop Improvement, Faculty of Sciences of Sfax, University of Sfax, 3038 Sfax, Tunisia; Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, F-63000 Clermont-Ferrand, France.
Microalgae like Chlorella can switch to mixotrophic growth using organic carbon sources found in olive mill wastewaters. This metabolic shift involves changes in protein expression, potentially aiding in wastewater detoxification.
Area of Science:
- * Biotechnology and Environmental Microbiology
- * Microalgal Physiology and Metabolism
Background:
- * Microalgae species, such as Chlorella, can utilize organic carbon for growth, exhibiting mixotrophic metabolism under light and organic substrate conditions.
- * Olive mill wastewaters (OMWWs) present a potential carbon source for microalgae, but require pretreatment, such as laccase treatment, for effective utilization.
Purpose of the Study:
- * To investigate the metabolic and proteomic shifts in Chlorella sp. when grown in photoautotrophy versus mixotrophy using pretreated OMWWs.
- * To analyze the functional role of specific proteins, including an ammonium transporter, in OMWWs detoxification and microalgal adaptation.
Main Methods:
- * Comparative proteomic and transcriptomic analyses of Chlorella sp. under photoautotrophic and mixotrophic conditions.
- * Bioinformatic analysis utilizing UniProt, InterPro, KEGG Pathway, and Gene Ontology databases.
- * Homology modeling to investigate the three-dimensional structure and potential function of the ammonium transporter protein.
Main Results:
- * Mixotrophic growth in OMWWs led to the overexpression of cell cycle, signaling, transport, and chaperone proteins.
- * A decrease in photosynthesis and carbohydrate/lipid metabolic pathways was observed under mixotrophy.
- * Upregulation of a specific ammonium transporter protein was detected, suggesting a role in OMWWs detoxification, with structural analysis indicating a functional trimeric form.
Conclusions:
- * Chlorella sp. exhibits significant metabolic and proteomic reprogramming when adapting to mixotrophic growth in OMWWs.
- * The identified ammonium transporter protein is a key player in OMWWs detoxification and warrants further investigation for its regulatory mechanisms.
- * This study highlights the potential of microalgae for bioremediation and valorization of industrial wastewaters.
