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Related Experiment Video

Updated: May 12, 2025

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

Sequence-encoded intermolecular base pairing modulates fluidity in DNA and RNA condensates.

Sumit Majumder¹, Sebastian Coupe^2,3, Nikta Fakhri³

¹Whitehead Institute for Biomedical Research, Cambridge, MA, USA.

Nature Communications

|May 7, 2025

Summary

This summary is machine-generated.

Researchers created programmable DNA and RNA liquids by controlling molecular interactions. This breakthrough allows precise tuning of fluid properties, advancing the study of cellular compartments and bottom-up material design.

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Area of Science:

Biomaterials Science
Molecular Biology
Rheology

Background:

Nature utilizes bottom-up self-assembly for complex structures.
Bridging molecular interactions and macroscopic properties is a key scientific challenge.
Understanding cellular liquid-like compartments requires adaptable model systems.

Purpose of the Study:

To develop programmable nucleic acid-based liquids.
To investigate the relationship between molecular hybridization and macroscopic rheological properties.
To create a platform for bottom-up programmable fluid generation.

Main Methods:

Condensing DNA and RNA using multivalent cations.
Utilizing sequence-specific hybridization for cross-linking.
Measuring molecular diffusivity and viscosity.

Main Results:

Programmable DNA and RNA liquids were successfully generated.
Inter-molecular hybridization directly correlated with reduced chain dynamics.
Macroscopic properties like viscosity and diffusivity were precisely controlled via hybridization energy.
Properties were modulated over several orders of magnitude through sequence design.

Conclusions:

Sequence-specific hybridization in condensed nucleic acids dictates macroscopic rheological behavior.
This work provides a robust platform for creating programmable fluids.
The findings offer insights into the formation and function of liquid-like compartments in biological cells.