Related Experiment Video
Updated: Jul 6, 2026
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
Multi-drug-resistant bacteria on household textiles: characteristics, transmission and low-temperature washing
Nina Čuk1, Manca Lunder2, Brigita Tomšič1
1Faculty of Natural Sciences and Engineering, University of Ljubljana, Ljubljana, Slovenia.
Abstract:
The aim of this study was to analyse how the properties of reference-resistant bacterial strains and textiles affect the transmission of bacteria, the antibacterial potential of detergents and disinfectants and how this can be implemented in low-temperature washing. Bacterial cells were characterised by a hydrophobicity test, and textiles were characterised by roughness and surface energy. Transmission and suspension tests were then carried out, followed by an evaluation of the wash. The results show that cotton has the highest roughness, polyester is the most hydrophobic and MRSA (methicillin-resistant Staphylococcus aureus) is the most hydrophobic bacterium. It was observed that resistant bacteria can be transmitted to a considerable extent via household textiles. The suspension test showed that the 0.5% European Colourfastness Establishment (ECE) reference detergent did not achieve a sufficient reduction (>5 log colony forming units - CFU) for any of the strains tested, whereas the addition of 3% hydrogen peroxide or 4% quaternary ammonium silicone compound did so for all of them. Washing at 60 °C reduced the textile contamination sufficiently (>4 log CFU) for all strains tested, while the criteria for cross-contamination and wash water were not met (<1.1 CFU/mL). However, the addition of disinfectants to the detergent at 30 °C met all hygiene requirements with the complete elimination of all tested bacteria on all textiles. Washing home textiles contaminated with resistant bacteria at low temperatures was only possible if a disinfectant was added. Otherwise, textiles can be an important vehicle for the transmission of resistant bacteria in domestic facilities.
Related Concept Videos
00:57Antibiotic Selection
01:33Bacterial Transformation
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
01:30Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
01:29Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
01:25Transmission-based Precautions II: Airborne and Protective Environment
Airborne precautions:
Use airborne precautions when treating patients known or suspected to have diseases that spread through the air—for example, tuberculosis or measles. These organisms are present in smaller droplets expelled by an infected person and...
01:18Surface Membrane Barriers
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...