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Toward handheld optically guided biopsy combining diffuse reflectance spectroscopy and autofluorescence.

Lotte M de Roode1, Simon T Sørensen2, Stefan D van der Stel1

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|May 8, 2025
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This study validates a new optical spectroscopy system for liver biopsy. The technology accurately distinguishes cancerous tissue from healthy liver, improving diagnostic accuracy for percutaneous liver biopsies.

Keywords:
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Area of Science:

  • Medical devices
  • Biotechnology
  • Optical spectroscopy

Background:

  • Percutaneous liver biopsy is crucial for diagnosing liver conditions.
  • Enhancing diagnostic yield and accuracy in liver biopsies remains a clinical challenge.
  • Current methods may lack precision in differentiating tumorous from healthy liver tissue.

Purpose of the Study:

  • To validate optical spectroscopy technologies for a handheld, optically guided biopsy device.
  • To improve the diagnostic accuracy of percutaneous liver biopsy procedures.
  • To develop a system for real-time classification of liver tissues during biopsy.

Main Methods:

  • A fiber-optic needle probe combining diffuse reflectance spectroscopy (DRS) and autofluorescence (AF) spectroscopy was designed and constructed.
  • Miniature spectrometers were integrated for data acquisition.
  • Linear discriminant analysis was applied to DRS and AF data from excised liver tissues (healthy and metastatic colorectal cancer).

Main Results:

  • The miniaturized DRS and AF spectroscopy system demonstrated high accuracy in distinguishing tumors from healthy liver tissue.
  • Sensitivity reached 95% and specificity reached 96%.
  • The system was validated on excised liver segments, supporting its potential for in vivo application.

Conclusions:

  • The feasibility of miniaturizing a combined DRS and AF spectroscopy system for tissue classification was successfully demonstrated.
  • This optical technology supports the development of a handheld, optically guided biopsy device.
  • The validated system has the potential to enhance the diagnostic capabilities of liver biopsy procedures.