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Published on: June 30, 2023
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Protocol for detecting 3D dermal fibroblast cellular dynamics in mice using an intravital imaging technique
Toru Hiratsuka1, Emanuel Rognoni2
1Department of Oncogenesis and Growth Regulation, Research Center, Osaka International Cancer Institute, Chuoku, Osaka 541-8567, Japan; Department of Molcular Oncology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
STAR Protocols
|May 8, 2025
Summary
This study introduces a new intravital imaging protocol to observe 3D dermal fibroblast dynamics in live mice. This method allows for detailed analysis of cellular behaviors crucial for skin health and disease.
Area of Science:
- Dermatology
- Cell Biology
- Biomedical Imaging
Background:
- Dermal fibroblasts are key to skin extracellular matrix production and remodeling.
- Distinct fibroblast subpopulations have varied roles in skin homeostasis, wound healing, and diseases like fibrosis and cancer.
Purpose of the Study:
- To present a protocol for detecting 3D dermal fibroblast cellular dynamics in mice.
- To enable visualization without the need for tissue fixation.
Main Methods:
- Intravital imaging technique applied to live mice.
- Detailed procedures for sample preparation, labeling, mounting, imaging, and recovery.
- Quantification of cellular dynamics.
Main Results:
- Successful implementation of a protocol for intravital imaging of dermal fibroblasts.
- Demonstration of a method to study cellular dynamics in 3D without tissue fixation.
- Provides a framework for analyzing fibroblast behavior in vivo.
Conclusions:
- The developed protocol facilitates the study of dermal fibroblast cellular dynamics in live mice.
- This technique offers new insights into fibroblast functions during skin homeostasis and disease.
- Enables advanced research in skin biology and pathology.
