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Updated: May 15, 2025
07:15
Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
Published on: August 23, 2024
297
Muscone Attenuates Uveitis Through the PI3K/AKT Signaling Pathway
Xianyang Liu1,2, Hangjia Zuo2, Chao Wu1
1Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing, China.
Investigative Ophthalmology & Visual Science
|May 9, 2025
Summary
Muscone effectively treats experimental autoimmune uveitis (EAU) by reducing inflammation and restoring the blood-retinal barrier. This novel therapeutic agent modulates the PI3K-AKT signaling pathway for its anti-inflammatory effects.
Area of Science:
- Ophthalmology
- Immunology
- Pharmacology
Background:
- Uveitis is a vision-threatening, immune-mediated ocular disorder.
- The blood-retinal barrier (BRB) hinders drug delivery, complicating uveitis treatment.
- Novel therapeutic strategies are needed to effectively manage uveitis.
Purpose of the Study:
- To identify a novel therapeutic agent for uveitis.
- To investigate the mechanism of action of potential uveitis treatments.
- To evaluate the efficacy of Muscone in an experimental autoimmune uveitis (EAU) model.
Main Methods:
- Experimental autoimmune uveitis (EAU) was induced in mice.
- Blood-retinal barrier integrity was assessed using Evans blue and fundus fluorescein angiography.
- Inflammatory responses were evaluated via histology and in vivo proteomics.
- The PI3K-AKT signaling pathway was investigated as a potential mechanism.
Main Results:
- Muscone significantly alleviated EAU and restored BRB integrity.
- Muscone downregulated inflammatory factors in retinal and BV2 cells.
- Proteomic analysis revealed Muscone modulates the PI3K-AKT pathway.
- Inhibition of PI3K with LY294002 reduced EAU inflammatory phenotype and BRB destruction.
Conclusions:
- Muscone demonstrates protective efficacy against EAU progression.
- Muscone's therapeutic action involves the modulation of the PI3K-AKT signaling pathway.
- This study provides mechanistic insights into Muscone's anti-inflammatory effects in uveitis.
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