Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Signaling in Plants01:25

Cell Signaling in Plants

5.4K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.4K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

4.9K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
4.9K
Plant Hormones01:56

Plant Hormones

23.1K
Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.
23.1K
Short-distance Transport of Resources02:12

Short-distance Transport of Resources

15.6K
Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
15.6K
Notch Signaling Pathway03:14

Notch Signaling Pathway

4.1K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.1K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stapokibart provides significant improvements in signs and symptoms of atopic dermatitis irrespective of prior systemic treatment: a post-hoc analysis of a phase 3 trial.

The Journal of dermatological treatment·2026
Same author

Dermatitis Artefacta: A Multidisciplinary Approach.

The American journal of case reports·2026
Same author

Social isolation, loneliness, genetic susceptibility, and the risk of hypothyroidism.

Social neuroscience·2026
Same author

Advancing synthetic biology with engineered chemically inducible gene regulatory systems.

Biotechnology advances·2026
Same author

Correction: Exploring the role of ferroptosis in pemphigus: identification of diagnostic markers and regulatory mechanisms.

Frontiers in medicine·2026
Same author

A Planarity-Hindrance Co-Balance Strategy to Develop Antiparallel H-Aggregates With Minimal Absorbance Blueshift for Type I Photodynamic Therapy.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

Crosstalk Regulation Between Vesicle Trafficking and Ion Transport in Plant Response to Abiotic Stresses.

Plant, cell & environment·2026
Same journal

Rethinking Acylsugar-Based Resistance: Breaking the Growth-Defense Trade-Off Through Precision Metabolic Engineering.

Plant, cell & environment·2026
Same journal

MALDI-MSI: Insights Into Plant, Cell and Environment.

Plant, cell & environment·2026
Same journal

Increasing Ascorbate Content of Non-Heading Chinese Cabbage Using Gene Editing.

Plant, cell & environment·2026
Same journal

DNA Methylation-Mediated Reprogramming of Gene Expression and Flowering Under Combined Heat and Drought Stress in Maize.

Plant, cell & environment·2026
Same journal

Automated Phenotyping Unveils Trait-Specific Genotypic Variation in Arbuscular Mycorrhiza Responsiveness in Maize (Zea mays).

Plant, cell & environment·2026
See all related articles

Related Experiment Video

Updated: May 12, 2025

Lateral Root Inducible System in Arabidopsis and Maize
09:23

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

13.7K

A CLE14 Signalling Cascade Promotes Arabidopsis Root Hair Elongation.

Yong Zhou1, Youxin Yang2, Ziwen Qiu1

  • 1College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China.

Plant, Cell & Environment
|May 9, 2025
PubMed
Summary
This summary is machine-generated.

Arabidopsis CLE14 peptide is crucial for root hair elongation. It signals through CLV2/CRN and ethylene pathways, activating RHD6 and RSL4 to promote growth via hydrogen peroxide and nitric oxide.

Keywords:
CLE14 peptideCLV2CRNH2O2NORHD6RSL4ethyleneroot hair

More Related Videos

Author Spotlight: Optimizing Hairy Root-Based Transformation Protocols for Enhanced Efficiency in Brassicaceae
08:52

Author Spotlight: Optimizing Hairy Root-Based Transformation Protocols for Enhanced Efficiency in Brassicaceae

Published on: December 22, 2023

3.3K
Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
08:54

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

9.7K

Related Experiment Videos

Last Updated: May 12, 2025

Lateral Root Inducible System in Arabidopsis and Maize
09:23

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

13.7K
Author Spotlight: Optimizing Hairy Root-Based Transformation Protocols for Enhanced Efficiency in Brassicaceae
08:52

Author Spotlight: Optimizing Hairy Root-Based Transformation Protocols for Enhanced Efficiency in Brassicaceae

Published on: December 22, 2023

3.3K
Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
08:54

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

9.7K

Area of Science:

  • Plant biology
  • Molecular genetics
  • Developmental biology

Background:

  • CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION-RELATED (CLE) peptides regulate diverse plant processes.
  • Root hair elongation is vital for water and nutrient uptake.

Purpose of the Study:

  • To investigate the role of Arabidopsis CLE14 in root hair elongation.
  • To elucidate the molecular mechanism of CLE14-mediated root hair growth.

Main Methods:

  • CRISPR/Cas9 gene editing to generate cle14 mutants.
  • Overexpression of CLE14.
  • Application of synthetic CLE14 peptide.
  • Analysis of clv2 and crn mutants.
  • Ethylene signaling pathway analysis.
  • Gene expression analysis (RHD6, RSL4, EIN3, EIL1).
  • Measurement of hydrogen peroxide (H₂O₂) and nitric oxide (NO).

Main Results:

  • Arabidopsis CLE14 is a positive regulator of root hair elongation.
  • CLE14 function requires CLAVATA2 (CLV2) and CRINKLY4 (CRN) receptors.
  • Ethylene signaling acts downstream of the CLE14-CLV2/CRN module.
  • CLE14 upregulates RHD6 and RSL4 via EIN3/EIL1 transcription factors.
  • RSL4 triggers H₂O₂ and NO accumulation, promoting root hair elongation.

Conclusions:

  • CLE14, CLV2, and CRN form a signaling module regulating root hair elongation.
  • Ethylene, RHD6, RSL4, H₂O₂, and NO are key downstream components in this pathway.
  • This study reveals the intricate signaling network controlling root hair development.