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Updated: May 13, 2025
An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
Published on: September 20, 2022
Integrated widely targeted metabolomics and flavoromics reveal processing-driven dynamic changes in functional
Yiyun Lin1,2, Hui Ouyang1,2,3, Ruobing Li1,2
1National & Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia Ulmoides, College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, PR China.
Abstract:
This study employed a widely targeted metabolomics approach combined with flavoromics evaluation to systematically analyze dynamic metabolic variations under four processing techniques: fresh EUL (XY), traditionally dried EUL (HG), green tea processed EUL (GT), and black tea processed EUL (BT). A total of 1839 non-volatile metabolites and 289 volatile metabolites were identified. Key affected metabolites included amino acids (e.g., essential amino acids upregulated 90 % in HG), lipids (oxidized fatty acids elevated in BT), phenolic acids (23 % increase in GT), flavonoids (120+ downregulated in BT), nucleotides, and terpenoids. Each processing method demonstrates unique advantages: HG preserves amino acids, GT enhances catechin content, and BT optimizes secondary metabolites. This study provides the first comprehensive metabolic map of EUL processing, offering a scientific foundation for standardizing product quality and developing functional applications.
