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Related Experiment Videos

Calcium-dependent action potentials in guinea-pig olfactory cortex neurones.

M Galvan, A Constanti, P Franz

    Pflugers Archiv : European Journal of Physiology
    |July 1, 1985
    PubMed
    Summary

    Calcium-dependent action potentials in guinea pig olfactory neurons were studied. A novel TTX-insensitive inward current was identified, responsible for anomalous rectification and prolonged action potential plateaus.

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    Area of Science:

    • Neuroscience
    • Electrophysiology
    • Cell Biology

    Background:

    • Olfactory neurons generate action potentials crucial for signal transduction.
    • Tetrodotoxin (TTX)-sensitive sodium channels are typically responsible for action potential generation.
    • The role of calcium (Ca2+) and other ions in olfactory neuron excitability requires further elucidation.

    Purpose of the Study:

    • To investigate the ionic mechanisms underlying Ca2+-dependent action potentials in guinea pig olfactory neurons.
    • To characterize the properties of inward currents contributing to action potential generation and modulation.
    • To identify novel ion channels involved in olfactory neuron excitability.

    Main Methods:

    • Whole-cell patch-clamp recordings in guinea pig olfactory neurons.

    Related Experiment Videos

  • Voltage-clamp and current-clamp electrophysiology.
  • Pharmacological manipulation using TTX, Cs+, tetraethylammonium (TEA), Ba2+, Sr2+, Cd2+, and choline.

    • Main Results:

    • Observed Ca2+-dependent action potentials in TTX-treated olfactory neurons.
    • Identified anomalous rectification at subthreshold potentials and outward rectification at more positive potentials.
    • Demonstrated that intracellular Cs+ loading prolonged action potentials by blocking outward rectification.
    • Showed that external Ba2+ and Sr2+ could substitute for Ca2+ in supporting action potentials, while Mg2+ could not.
    • Identified a TTX-insensitive, slowly inactivating inward current responsible for anomalous rectification and prolonged spike plateau.

    Conclusions:

    • Olfactory neurons possess Ca2+-dependent action potentials mediated by a TTX-insensitive inward current.
    • This inward current contributes to subthreshold anomalous rectification and prolonged action potential plateau.
    • The findings reveal a novel mechanism of excitability in olfactory neurons potentially involving calcium channels.