Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lineage Commitment01:21

Lineage Commitment

2.9K
Commitment is the  process whereby stem cells:
2.9K
Notch Signaling Pathway03:14

Notch Signaling Pathway

4.1K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.1K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.0K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.0K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

2.6K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.6K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patient-derived organoids reveal ductal dysfunction and CFTR-modulator responses in chronic pancreatitis.

Cell stem cell·2026
Same author

Evaluating an Academic Health Department During a Pandemic: Lessons Learned.

Progress in community health partnerships : research, education, and action·2026
Same author

Artificial intelligence and academic anxiety? a meta-analysis.

BMC psychology·2026
Same author

αKG-mediated carnitine synthesis drives DNA repair via histone acetylation.

Nature·2026
Same author

MICU proteins facilitate calcium-dependent mitochondrial metabolon formation to regulate cellular energetics independently of MCU.

Nature metabolism·2026
Same author

Postmyocardial Infarction Depression as a Modifiable Secondary Prevention Risk Factor.

Cardiology in review·2026

Related Experiment Video

Updated: May 14, 2025

Culturing and Manipulation of O9-1 Neural Crest Cells
08:32

Culturing and Manipulation of O9-1 Neural Crest Cells

Published on: October 9, 2018

9.9K

A retinoic acid:YAP1 signaling axis controls atrial lineage commitment.

Elizabeth Abraham1, Aleksandra Kostina2, Brett Volmert2

  • 1Department of Cardiovascular Sciences, Aging + Cardiovascular Discovery Center, Lewis Katz School of Medicine, Temple University, Philadelphia, PA 19140, USA.

Cell Reports
|May 9, 2025
PubMed
Summary

Retinoic acid (RA) signaling directs cardiac progenitor cells toward an atrial fate. The Hippo pathway effector YAP1 collaborates with RA signaling to activate atrial enhancers and promote atrial chamber development.

Keywords:
CP: Developmental biologyNR2F2SHFTEAD4YAP1atrial differentiationcardiac enhancerscardiac progenitorschamber developmentheart organoidsretinoic acid signaling

More Related Videos

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies
09:04

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

Published on: April 22, 2017

8.9K
Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
10:32

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

10.7K

Related Experiment Videos

Last Updated: May 14, 2025

Culturing and Manipulation of O9-1 Neural Crest Cells
08:32

Culturing and Manipulation of O9-1 Neural Crest Cells

Published on: October 9, 2018

9.9K
Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies
09:04

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

Published on: April 22, 2017

8.9K
Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
10:32

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

10.7K

Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Cardiovascular Research

Background:

  • Retinoic acid (RA) signaling is crucial for inducing atrial lineage gene expression and cell fate in cardiac progenitor cells (CPCs).
  • The precise regulatory network downstream of RA signaling that governs this process is not fully understood.

Purpose of the Study:

  • To identify novel downstream effectors of RA signaling in human embryonic stem cell (hESC)-derived CPCs.
  • To elucidate the role of the Hippo pathway, specifically YAP1 and TEAD4, in RA-mediated atrial lineage specification.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) and single-nucleus ATAC sequencing (snATAC-seq) were employed on hESC-derived CPCs.
  • Functional genomics approaches were used to analyze gene expression and chromatin accessibility.
  • Yap1 deletion in mouse embryos and hESC-derived heart organoid models were utilized for in vivo and in vitro validation.

Main Results:

  • Unbiased analysis identified YAP1 and TEAD4 as key Hippo pathway effectors integrated into the atrial transcription factor network.
  • YAP1 was shown to activate RA-responsive enhancers in CPCs, promoting the expression of RA-induced genes like Nr2f2.
  • Yap1 deletion in mouse embryos impaired RA-induced gene expression in second heart field CPCs, and YAP1 regulated atrial chamber formation in hESC-derived organoids.

Conclusions:

  • YAP1 functions as a critical mediator of RA signaling in the induction of atrial lineages during cardiogenesis.
  • YAP1 cooperates with RA signaling to establish atrial cell fate and regulate atrial chamber development, but is dispensable for ventricle formation.