Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inflammatory Response01:28

Inflammatory Response

1.6K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
1.6K
Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

927
Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
927

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PTPN1/PTPN2 inhibition improves NK cancer therapy by enhancing IL-2 and mitigating TGFβ1 responses.

EMBO reports·2026
Same author

Human APOB-expressing mice translate molecular phenotypes across the Alzheimer's disease spectrum.

Brain, behavior, and immunity·2026
Same author

Differential <i>cis</i>-regulatory regions of KS dehydrins of Arctic and temperate <i>Oxytropis</i> spp.

Genome·2026
Same author

Uncovering the individual immunotherapeutic roles of PTPN1 and PTPN2 in T cells during dual inhibition.

iScience·2025
Same author

Leishmania donovani's protein tyrosine phosphatases interact with DUF21 and respond to environmental magnesium.

The FEBS journal·2025
Same author

Impact of culture vessel materials on biomanufacturing of dendritic cell-based immunotherapies in closed systems.

Biomaterials science·2025

Related Experiment Video

Updated: May 12, 2025

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays
08:37

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Published on: April 18, 2025

99

PTPN2 Negatively Regulates Macrophage Immune Responses and Cellular Bioenergetics.

Valerie Vinette1,2, Yevgen Zolotarov1,2, Alexandre Poirier2,3

  • 1Department of Biochemistry, McGill University, Montreal, Canada.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|May 9, 2025
PubMed
Summary

Protein tyrosine phosphatase N2 (PTPN2) regulates macrophage inflammation and metabolism. Inhibiting PTPN2 enhances anti-cancer immune responses by boosting macrophage pro-inflammatory activity and altering mitochondrial function.

Keywords:
LysM‐CrePTPN1PTPN2macrophagesproinflammationprotein tyrosine phosphatases

More Related Videos

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
07:45

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

35.3K
Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line
06:38

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Published on: August 2, 2021

26.5K

Related Experiment Videos

Last Updated: May 12, 2025

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays
08:37

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Published on: April 18, 2025

99
Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
07:45

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

35.3K
Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line
06:38

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Published on: August 2, 2021

26.5K

Area of Science:

  • Immunology
  • Cancer Therapeutics
  • Cell Metabolism

Background:

  • Protein tyrosine phosphatase N2 (PTPN2) is a key regulator of cytokine signaling and macrophage differentiation.
  • Inhibition of PTPN2 and PTPN1 (PTP1B) is a promising cancer immunotherapy strategy.
  • The precise role of PTPN2 in macrophage immune and metabolic functions requires further investigation.

Purpose of the Study:

  • To investigate the function of PTPN2 in regulating macrophage polarization and activity.
  • To elucidate PTPN2's role in macrophage immune and metabolic responses.

Main Methods:

  • Utilized two distinct mouse models to specifically inhibit PTPN2 expression in macrophages.
  • Employed a chemical inhibitor with a human macrophage cell line.
  • Assessed immune and metabolic profiles of macrophages with altered PTPN2 expression.

Main Results:

  • PTPN2 ablation in macrophages altered their immunometabolic transcriptome.
  • Macrophage PTPN2 deficiency enhanced pro-inflammatory responses, increasing IFN-ɣ and nitric oxide production.
  • PTPN2 deficiency disrupted mitochondrial respiration, decreasing oxygen consumption and ATP production.

Conclusions:

  • PTPN2 acts as a dampener of the macrophage pro-inflammatory response.
  • PTPN2 influences macrophage mitochondrial respiration.
  • Inhibiting PTPN2 in macrophages contributes to the efficacy of combined PTPN1 and PTPN2 inhibition in cancer therapy.