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High-Throughput Fluorescence Screening Enables Globally Consistent Identification of ABA Signaling Modulators.

Yang-Yang Gao1, Chang-Xin Yang1, Hong Wu1

  • 1State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang, 550025, P. R. China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|May 9, 2025
PubMed
Summary

A new high-throughput screening platform efficiently identifies plant stress modulators. This method discovered Diopyridin, which protects plants from drought by regulating abscisic acid (ABA) signaling.

Keywords:
ABA signaling modulatorsGhitFluors platformfluorescent chemosensorglobal high‐throughput screeninglebactin

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Area of Science:

  • Plant Biology
  • Biochemistry
  • Environmental Science

Background:

  • Environmental stresses significantly reduce crop yields, necessitating strategies for plant adaptation.
  • Abscisic acid (ABA) signaling pathways, particularly ABA receptors, are critical for plant stress response.
  • Efficiently identifying modulators of ABA signaling is a key challenge in plant science.

Purpose of the Study:

  • To establish a global high-throughput fluorescence screening (GhitFluors) platform for identifying ABA signaling modulators.
  • To validate the efficacy of the GhitFluors platform in identifying compounds with significant biological activity.

Main Methods:

  • Development and implementation of the GhitFluors platform for large-scale screening.
  • Assessment of binding affinity of identified compounds using classical methods.
  • Evaluation of inhibitory activity against key ABA signaling components like HAB1.
  • Testing the efficacy of identified compounds in mitigating drought stress in plants.

Main Results:

  • The GhitFluors platform demonstrated reliable binding affinity comparable to classical methods.
  • Diopyridin was identified as a potent ABA signaling modulator with strong binding to PYR1.
  • Diopyridin exhibited over 80% inhibitory activity against HAB1, similar to ABA.
  • Diopyridin effectively alleviated drought stress effects in plants.

Conclusions:

  • The GhitFluors platform offers a highly efficient method for identifying ABA signaling modulators.
  • Diopyridin represents a promising compound for enhancing plant stress tolerance.
  • This work heralds a new era in the discovery of plant stress adaptation regulators.