Portable and intelligent ratio fluorometry and colorimetry for dual-mode detection of dopamine based on B, N-codoped...

A dual-mode approach was developed for dopamine (DA) assay based on boron (B) and nitrogen (N) co-doped carbon dots (B, N-CDs). This platform enabled highly sensitive and specific detection of DA in biological samples through collaborative ratio fluorometry and colorimetry. B, N-CDs were synthesized via a one-pot hydrothermal method using 3-aminobenzylboric acid (3-APBA) and proline (Pro) as precursors. B, N-CDs exhibited dark blue fluorescence under ultraviolet (UV) light excitation with a quantum yield (QY) of 0.05. In the presence of DA, the fluorescence of B, N-CDs displayed bright blue with the QY at 0.22. The original fluorescence peak intensity at 420 nm of B, N-CDs decreased, while a new peak at 465 nm increased significantly with the increasing concentration of DA. In this case, a ratio fluorometry for DA detection was constructed using B, N-CDs as fluorescence probes. On the other way, the color of B, N-CDs solution changed from colorless to brown with the addition of DA. A colorimetry for DA sensing was established based on the absorbance enhancement of B, N-CDs. There is excellent linear relationship within the concentration range of 2.5-500 microM for DA sensing with limit of detection (LOD) at 0.22 microM (ratio fluorometry) and 1.04 microM (colorimetry) and the relative standard deviations (RSD%) are 0.2170 and 0.02131, respectively. To enable the real-time visual and portable quantification of DA, two intelligent methods were explored by a program named RGB color analysis in a smartphone and machine learning. This dual-mode sensing strategy combined high sensitivity, wide linear range and ease of operation, offering a novel solution for rapid analysis of DA in complex biological substrates.