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Development of a new L-fucose purity certified reference material
Weizhu Chen1, Hua Fang1, Wenhui Jin1
1Engineering Research Center of Marine Biological Resource Comprehensive Utilization,Third Institute of Oceanography, Ministry of Natural Resource, Xiamen, Fujian 361005, PR China; Xiamen Ocean Vocational College, Xiamen, Fujian 361100, PR China; Fujian Provincial Key Laboratory of Island Conservation and Development (Island Research Center, MNR), Pingtan, Fujian 350400, PR China.
A new L-fucose purity Certified Reference Material (CRM) was developed for accurate L-fucose content determination. This reliable CRM ensures high accuracy and metrological traceability in analytical methods.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Reference Materials
Background:
- Certified Reference Materials (CRMs) are crucial for accurate equipment calibration and analytical method validation.
- L-fucose, a natural sugar with significant biological functions, has diverse applications in medicine, food, and cosmetics.
- Accurate quantification of L-fucose necessitates a dependable CRM.
Purpose of the Study:
- To develop a novel L-fucose purity Certified Reference Material (CRM) adhering to ISO principles.
- To characterize the CRM through structural analysis, homogeneity testing, and stability evaluation.
- To establish the certified value and associated uncertainty for the L-fucose CRM.
Main Methods:
- Utilized the mass balance (MB) approach to quantify the main component, moisture, and volatile/nonvolatile impurities.
- Employed quantitative proton nuclear magnetic resonance (q¹H NMR) for CRM characterization.
- Identified the primary component (PC) using high-performance liquid chromatography with ultraviolet detection (HPLC-UV) and pre-column derivatization.
Main Results:
- The L-fucose purity CRM demonstrated stability for 18 months at ambient temperatures and 30 days at 50 °C.
- The CRM was found to be adequately homogeneous.
- The certified value was determined to be 99.5 ± 1.2% at a 95% confidence level (k=2).
Conclusions:
- A reliable L-fucose purity CRM has been successfully developed.
- The CRM meets requirements for accuracy and metrological traceability.
- This CRM will support precise L-fucose quantification across various industries.
