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Updated: May 12, 2025

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Knockdown of RRBP1 regulates endoplasmic reticulum stress to mitigate malignant progression and suppress bortezomib

Jie Wang1, Haipeng Jia1, Sulong Lv1

  • 1Department of Hematology, The Second Affiliated Hospital of Shandong First Medical University, No. 366, Taishan Street, Taishan District, Tai'an City, Shandong Province 271000, China.

Molecular Immunology
|May 9, 2025
PubMed
Summary
This summary is machine-generated.

Ribosome binding protein 1 (RRBP1) knockdown inhibits multiple myeloma (MM) cell proliferation, migration, and invasion. This suggests RRBP1 is a potential therapeutic target for treating MM.

Keywords:
Drug resistanceEndoplasmic reticulum stressGRP78Multiple myelomaRRBP1

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Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Ribosome binding protein 1 (RRBP1) is implicated in cancer progression.
  • RRBP1 is linked to poor prognosis and drug resistance in multiple myeloma (MM).
  • The precise mechanism of RRBP1 in MM remains unclear.

Purpose of the Study:

  • To investigate the role and mechanism of RRBP1 in multiple myeloma (MM) cells.
  • To explore RRBP1 as a potential therapeutic target for MM.

Main Methods:

  • Immunohistochemistry, qRT-PCR, and Western blot were used to assess RRBP1 and GRP78 expression.
  • Cell viability, proliferation, apoptosis, migration, and invasion assays were performed.
  • Gene knockdown and overexpression techniques were employed to study RRBP1 and GRP78 functions.

Main Results:

  • RRBP1 and GRP78 were upregulated in MM tissues and cells.
  • RRBP1 knockdown reduced MM cell proliferation, migration, and invasion, and decreased GRP78 expression and endoplasmic reticulum stress.
  • RRBP1 inhibition enhanced MM cell apoptosis and increased sensitivity to bortezomib in resistant cells, with GRP78 overexpression partially reversing these effects.

Conclusions:

  • RRBP1 knockdown exhibits an inhibitory effect on multiple myeloma cells.
  • RRBP1 represents a promising novel therapeutic target for MM treatment.