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Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
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Condensins02:15

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The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
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The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
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Cohesins02:20

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Condensins02:15

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Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...

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Related Experiment Video

Updated: May 12, 2026

Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo
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Published on: July 15, 2016

Cohesin in 3D: development, differentiation, and disease.

Maria Solé-Ferran1, Ana Losada2

  • 1Chromosome Dynamics Group, Molecular Oncology Programme, Spanish National Cancer Research Centre (CNIO), Madrid 28029, Spain.

Genes & Development
|May 9, 2025
PubMed
Summary

Cohesin is vital for organizing DNA and ensuring accurate cell division during embryonic development. Dysfunction in cohesin leads to developmental disorders known as cohesinopathies.

Keywords:
Cornelia de Lange syndromeSMCchromosome organizationcohesinopathiesembryogenesismouse models

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Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Cohesin plays a critical role in genome spatial organization and sister chromatid cohesion.
  • These functions are essential for accurate chromosome segregation, DNA repair, and gene expression regulation.
  • Cohesin's functions are particularly crucial during embryonic development, enabling the formation of complex organisms.

Purpose of the Study:

  • To summarize current understanding of cohesin's mechanisms in chromatin looping and cohesion.
  • To review the role of cohesin and its factors in cell differentiation and embryonic development.
  • To discuss cohesinopathies, developmental syndromes resulting from cohesin dysfunction.

Main Methods:

  • Literature review of cohesin function in genome organization.
  • Review of studies on cohesin's role in cell differentiation and embryogenesis.
  • Discussion of clinical aspects of cohesinopathies.

Main Results:

  • Cohesin facilitates chromatin looping and sister chromatid cohesion, impacting genome structure.
  • Cohesin is integral to processes governing cell differentiation and overall embryonic development.
  • Defects in cohesin function are linked to a class of human developmental disorders.

Conclusions:

  • Cohesin is a fundamental protein complex essential for normal embryonic development.
  • Understanding cohesin's functions provides insights into developmental processes and diseases.
  • Cohesinopathies highlight the critical importance of cohesin in human development.