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Updated: May 12, 2025

A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy
Knowledge and practices in transfusion regarding bedside compatibility test: Insights from a monocentric
Ikbel Ghachem1,2, Dorra Baccouche2, Mohamed Yassine Kaabar2,3
1Faculty of Medecine of Tunis, University of Tunis El Manar, Tunisia.
Background:
The bedside compatibility test (BCT) is the final barrier to transfusion safety, aiming to prevent ABO-incompatible incidents. This study evaluated the knowledge and practices of BCT and assessed the impact of a training session introducing a new technique of BCT based on Serafol ABO card.
Methods:
This is an observational study targeting transfusion-qualified staff working at the MTM hospital in Nabeul. It involved an initial knowledge assessment, a training session on BCT principles, and post-assessment using an anonymous questionnaire that included the interpretation of Serafol ABO card examples.
Results:
Among 93 participants, 18% frequently performed transfusions, and 31% had prior training. Incorrect BCT practices, including unsafe serum methods, were noted in 46%. Only 7.5% completed necessary pre-transfusion checks. The frequency of overall scores (≥50/100) increased significantly from 43% to 90% (p = 0.000) after the training session. Correct interpretation of the Serafol ABO card was achieved by 86% and 81% of participants in isogroup and non-isogroup compatible situations, respectively. However, 20.5% of participants would have transfused an incompatible unit. A significant difference was observed between doctors and paramedics in interpreting non-isogroup compatible and incompatible situations (p = 0.03). The overall mean interpretation score was 7.7/9, with a 77% compatibility accuracy rate. Professional profile parameters did not significantly influence correct responses.
Conclusion:
This study revealed gaps in BCT practices, significantly improved through training and the introduction of the Serafol ABO card. Continuous training, medical assessments, and procedure monitoring are vital to enhance transfusion safety.
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