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Updated: May 12, 2025

A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy
Knowledge and practices in transfusion regarding bedside compatibility test: Insights from a monocentric
Ikbel Ghachem1,2, Dorra Baccouche2, Mohamed Yassine Kaabar2,3
1Faculty of Medecine of Tunis, University of Tunis El Manar, Tunisia.
Training on the Serafol ABO card significantly improved bedside compatibility testing (BCT) knowledge and practices, enhancing transfusion safety. This intervention addressed critical gaps in preventing ABO-incompatible events.
Area of Science:
- Transfusion Medicine
- Patient Safety
- Clinical Laboratory Science
Background:
- Bedside compatibility testing (BCT) is crucial for preventing ABO-incompatible transfusions.
- Existing BCT knowledge and practices among healthcare professionals require evaluation.
- The Serafol ABO card offers a new technique for BCT.
Purpose of the Study:
- To assess the current knowledge and practices of bedside compatibility testing.
- To evaluate the impact of a training session on BCT using the Serafol ABO card.
- To identify areas for improvement in transfusion safety protocols.
Main Methods:
- An observational study was conducted with transfusion-qualified staff.
- Initial and post-training knowledge assessments were performed using anonymous questionnaires.
- The assessment included interpretation of Serafol ABO card examples.
Main Results:
- Significant improvement in overall BCT scores from 43% to 90% post-training (p=0.000).
- High correct interpretation rates for the Serafol ABO card (86% isogroup, 81% non-isogroup).
- Despite improvements, 20.5% of participants would have transfused an incompatible unit, highlighting persistent risks.
Conclusions:
- Training and the Serafol ABO card significantly enhance BCT practices and knowledge.
- Continuous education, medical assessments, and monitoring are essential for sustained transfusion safety.
- Addressing identified gaps in BCT is vital to minimize transfusion errors.
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