Related Experiment Video

Updated: May 12, 2025

Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
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Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.

Published on: April 5, 2018

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Leveraging Sortase A Electrostatics for Powerful Transpeptidation Reactions

Chen Wang1,2, Rémi Desmet1, Benoît Snella1

  • 1Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, Lille, F-59000, France.

Angewandte Chemie (International Ed. in English)
|May 9, 2025
PubMed
Summary

This study enhances protein engineering using sortase A pentamutant (SrtA-5M) by incorporating charged modules. This electrostatic assistance improves transpeptidation efficiency and broadens its application scope in biochemistry.

Keywords:
Electrostatic assistanceProtein engineeringProtein synthesisSortase ATranspeptidation

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