Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Micro-cold-forming: a simple, rapid, and inexpensive method for the fabrication of microcavities for 3D cell culture.

RSC advances·2026
Same author

Beyond integration: towards benchmarks for developmental potential in human stem cell-derived embryo models.

Human reproduction update·2026
Same author

Real-Time Monitoring of the Formation and Culture of Hybrid Cell-Microbiomaterial Spheroids Using Non-Faradaic Electrical Impedance Spectroscopy.

ACS biomaterials science & engineering·2025
Same author

Integration of Bioengineered Tools in Assisted Reproductive Technologies.

Advanced healthcare materials·2025
Same author

Monochorionic Twinning in Bioengineered Human Embryo Models.

Advanced materials (Deerfield Beach, Fla.)·2024
Same author

Nanofunctionalized Microparticles for Glucose Delivery in Three-Dimensional Cell Assemblies.

ACS applied materials & interfaces·2024

Related Experiment Video

Updated: Jun 12, 2025

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

12.6K

Morphometric-Assisted Prediction of Developmental Toxicity Using Stem Cell-Based Embryo Models in Microwells.

Vinidhra Shankar1, Athanasia Zoi Pappa1, Clemens van Blitterswijk1

  • 1MERLN Institute for Technology-Inspired Regenerative Medicine, Department of Cell Biology-Inspired Tissue Engineering (cBITE), Maastricht University, Maastricht, 6229ET, The Netherlands.

Advanced Healthcare Materials
|May 10, 2025
PubMed
Summary

This study introduces a novel stem cell model and automated platform to assess developmental toxicity. It reveals that certain compounds disrupt embryonic development by causing "morphotoxicity," even without impacting cell viability.

Keywords:
developmental and reproductive toxicity (DART)high throughput screeningmicrowellsstem cell‐based embryo modelsteratogens

More Related Videos

Using Chicken Embryo as a Powerful Tool in Assessment of Developmental Cardiotoxicities
11:00

Using Chicken Embryo as a Powerful Tool in Assessment of Developmental Cardiotoxicities

Published on: March 21, 2021

4.2K
In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
08:04

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

Published on: May 11, 2021

2.8K

Related Experiment Videos

Last Updated: Jun 12, 2025

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

12.6K
Using Chicken Embryo as a Powerful Tool in Assessment of Developmental Cardiotoxicities
11:00

Using Chicken Embryo as a Powerful Tool in Assessment of Developmental Cardiotoxicities

Published on: March 21, 2021

4.2K
In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
08:04

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

Published on: May 11, 2021

2.8K

Area of Science:

  • Developmental toxicology
  • Stem cell biology
  • High-throughput screening

Background:

  • Congenital abnormalities contribute significantly to fetal defects and mortality.
  • Animal studies face ethical and logistical constraints, driving the need for alternative models.
  • Existing in vitro models often lack complexity, automation, or real-time readouts.

Purpose of the Study:

  • To develop a scalable, automated platform for assessing chemical effects on early embryogenesis.
  • To introduce a novel embryo model, XEn/EpiCs, mimicking peri-implantation development.
  • To evaluate "morphotoxicity" as a complementary endpoint to cytotoxicity in developmental toxicity testing.

Main Methods:

  • Generation of a microwell-based embryo model (XEn/EpiCs) for co-development of extraembryonic endoderm and epiblast.
  • Utilizing a scalable, automated platform for imaging and quantifying morphological features.
  • Screening a library of 38 compounds to assess both cytotoxicity and morphotoxicity.

Main Results:

  • High doses of retinoic acid, caffeine, ampyrone, and dexamethasone significantly disrupted XEn/EpiC development.
  • Observed morphotoxic effects occurred independently of direct cytotoxicity in some cases.
  • The platform successfully quantified morphological changes indicative of developmental disruption.

Conclusions:

  • Morphotoxicity assessment provides crucial insights beyond traditional cytotoxicity assays.
  • This approach enhances the prediction of teratogenic risks from chemical exposures.
  • The developed platform and model offer a promising tool for developmental toxicity screening.