Related Experiment Video
Updated: May 13, 2025
Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
Published on: March 24, 2023
Protocol for in-plate staining of clinical peripheral blood mononuclear cell samples for high-throughput analysis via
Isabella Marciello1, Sonya Feng1, Ilana Gomez Diaz1
1Department of Medical Oncology, Center for Immuno-Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.
Abstract:
Clinical trial research relies on robust assays to monitor immune responses in patients. We present a protocol for optimization of in-plate staining for spectral flow cytometry tailored for high-throughput analysis of peripheral blood mononuclear cell (PBMC) samples from clinical research correlative studies. We describe steps for antibody titration, optimal in-plate staining, data acquisition on Sony ID7000 spectral cell analyzer, and validation of a flow cytometry assay.
