Decoding the effect of antibiotics on biofilm formation in biofilters

Biofilms have extensive applications and important roles in biological processes. This study aimed to investigate the effect and mechanism of low-concentration sulfamethoxazole (SMX) on biofilm development in biofilters. The effects of various SMX concentrations (0, 100 ng/L, 1000 ng/L) on microbial development were compared. Compared with the control group without SMX, the start-up period of R2 and R3 filters with SMX added was decreased by 9 % and 21 %, respectively. Under antibiotic stimulation, reactive oxygen species (ROS) and bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) concentrations increased, aligning with changes in extracellular polymer content and biofilm formation. Microbial community results showed that the presence of SMX promoted the growth of some manganese-oxidizing bacteria (MnOB), such as Massilia, Pedomicrobium, Sphingopyxis, Pseudomonas, and Bacillus. Functional gene analysis further revealed higher expression levels of genes related to c-di-GMP transformation in the presence of SMX. These findings suggest that microbial communities can adapt to their environment by accelerating biofilm formation at lower antibiotic concentrations. The results of this study provide new insights into the impact of low-concentration antibiotics on biofilm development and offer a crucial reference for biofilter design and optimization.