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Association between the gut microbiota and cystitis: A two-sample mendelian randomization study combined with the GEO
Zeyu Lin1, Ping Zhang2, Donghui Shi3
1The First School of Clinical Medicine, Nanjing Medical University, Nanjing, Jiangsu, 211166, China.
Microbial Pathogenesis
|May 10, 2025
Summary
This study used Mendelian randomization to investigate the gut microbiota
Area of Science:
- Microbiome Research
- Genetics
- Inflammatory Diseases
Background:
- Gut microbiota disturbances are linked to systemic inflammation and increased susceptibility to urinary tract infections like cystitis.
- The direct causal link between gut dysbiosis and cystitis pathogenesis remains incompletely understood.
Purpose of the Study:
- To elucidate the causal relationship between the intestinal microbiota and cystitis using Mendelian randomization (MR).
- To identify potential preventive and therapeutic targets for cystitis based on gut microbiota associations.
Main Methods:
- Genome-Wide Association Study (GWAS) summary statistics for cystitis (8,081 cases, 195,140 controls) and microbial taxa (18,340 individuals).
- Four MR methods (IVW, weighted medians, MR-Egger, random effects) and sensitivity analyses were employed.
- Metagenomic analysis, SNP-to-gene mapping, and differential gene expression (DEG) analysis were conducted for validation and mechanism exploration.
Main Results:
- Four gut microorganisms (RuminococcaceaeUCG011, Sutterella, Porphyromonadaceae, Veillonellaceae) were associated with reduced cystitis incidence.
- Four bacteria (Marvinbryantia, Odoribacter, Ruminiclostridium6, Sellimonas) were linked to an elevated risk of cystitis.
- Metagenomic analysis confirmed differential abundance of Sutterella and Odoribacter; ICAM1 and HP were identified as potential common genes.
Conclusions:
- Established genetic links between eight gut microbial components and cystitis.
- Identified two potential therapeutic gene targets (ICAM1, HP) for cystitis.
- Findings provide crucial insights into the gut-urinary tract axis and inform future research on cystitis etiology and management.
