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FERONIA interacts with NPL4 to regulate immunity gene mRNA nucleocytoplasmic transport in response to plant immunity
Li Guan1, Peilong Wang2, Yongliang Li1
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, PR China.
Abstract:
Nucleocytoplasmic transport plays a critical role in the activation of immune mechanisms in plant cells. Fluorescence imaging analysis indicated that a high concentration of rapid alkalinization factor (RALF) suppresses the immune response and can induce nuclear envelope (NE) shape deformation. This phenomenon depends on the receptor kinase FERONIA (FER). Consistently, bacterial infection also affects NE shape. This study presents evidence that FER displays functional interactions with NPL4 and that phosphorylated NPL4 promotes its stability. We reported the identification and characterization of the nuclear localization protein NPL4, which is directly involved in general mRNA nuclear export. FER and NPL4 mutations both affected rhizosphere Pseudomonas colonization levels, suggesting that the interactions between FER and NPL4 are largely indispensable for regulating rhizosphere Pseudomonas colonization levels. In addition, NPL4 altered the mRNA nucleocytoplasmic distribution of immune genes in conjunction with the function of RALF-FER in the suppression of plant immunity. In brief, NPL4, which is downstream of FER is required for innate immunity and mRNA nuclear accumulation of resistance genes in Arabidopsis. Overall, through the analysis of RALF-FER and the nuclear localization protein NPL4, this work provides novel insights into the mRNA nucleocytoplasmic transport of immune genes and plant health.
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