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Related Experiment Videos

Nonthrombogenic arterial reconstruction.

F F Senatore, E S Venkataramani, A K Lokapur

    Research Communications in Chemical Pathology and Pharmacology
    |July 1, 1985
    PubMed
    Summary
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    Heparin covalently bound to Human Umbilical Veins Grafts (HUVG) shows superior loading and stability compared to animal carotid arteries. This heparin-bound HUVG retains anticoagulative properties, promising for non-thrombogenic vascular prostheses.

    Area of Science:

    • Biomaterials Science
    • Vascular Surgery
    • Biomedical Engineering

    Background:

    • Heparin is crucial for preventing blood clots in vascular grafts.
    • Developing stable heparinized grafts is essential for small-caliber vascular prostheses.
    • Current methods for graft modification require optimization for enhanced performance.

    Purpose of the Study:

    • To covalently bind heparin to arterial reconstructions using EDC activation.
    • To evaluate the performance of ammonium hydroxide treated Human Umbilical Veins Grafts (HUVG) for heparin loading and stability.
    • To assess the anticoagulative activity of heparin-bound HUVG for potential use as a non-thrombogenic vascular prosthesis.

    Main Methods:

    • Carboxyl groups of heparin were activated using carbodiimide (EDC).

    Related Experiment Videos

  • Heparin was covalently attached to various arterial reconstructions, with a focus on ammonium hydroxide treated Human Umbilical Veins Grafts (HUVG).
  • In-vitro studies were conducted to assess heparin loading, stability, and anticoagulative activity.

    • Main Results:

    • Ammonium hydroxide treated HUVG demonstrated superior heparin loading and stability compared to goat, porcine, and canine carotid arteries.
    • The covalently bound heparin on HUVG retained its significant anticoagulative activity.
    • Heparin-bound HUVG shows potential for developing effective vascular prostheses.

    Conclusions:

    • Covalent binding of heparin to HUVG via EDC activation offers a stable and effective method for graft modification.
    • Heparinized HUVG exhibits promising anticoagulative properties, outperforming animal carotid arteries in key metrics.
    • This approach holds significant potential for the development of next-generation non-thrombogenic small-caliber vascular prostheses.