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New Morganella morganii Phage vB_Mm5 with Tolerance to Cu2+ Ions
Rusudan Goliadze1, Luka Kamashidze1, Natia Karumidze1,2
1Laboratory of General Microbiology, George Eliava Institute of Bacteriophages, Microbiology and Virology, Tbilisi, Georgia.
Background:
Research on phages targeting Morganella morganii is still emerging, with limited studies compared with other phage-host systems. Interest in these phages has increased due to rising antibiotic resistance and their potential for controlling M. morganii spread in the environment.
Materials And Methods:
This study investigates the biology and genetics of the novel M. morganii-infecting myophage vB_Mm5 and evaluates its stability under Cu2+ exposure.
Results:
Phage vB_Mm5 has a 10-min latent period and a burst size of 30 (±5). It shows high tolerance to elevated temperatures and Cu2+. The phage genome, comprising 163,232 bp dsDNA with 229 open reading frames, encodes genes that not only enhance the phage's predatory capabilities but also confer resistance to host defense mechanisms.
Conclusions:
vB_Mm5 is highly distinct from other sequenced M. morganii phages, does not contain any known virulence genes, and holds potential as a therapeutic agent against M. morganii infections.
Insights
A novel myophage, vB_Mm5, effectively targets Morganella morganii. This phage exhibits remarkable stability under heat and copper exposure, showing promise for combating antibiotic-resistant infections.
Area of Science:
- Microbiology
- Bacteriology
- Virology
Background:
- Research on phages targeting Morganella morganii is emerging.
- Increasing antibiotic resistance drives interest in phage therapy for M. morganii control.
Purpose of the Study:
- Investigate the biology and genetics of a novel M. morganii-infecting myophage, vB_Mm5.
- Evaluate the stability of vB_Mm5 under copper (Cu2+) exposure.
Main Methods:
- Characterization of myophage vB_Mm5 biology and genetics.
- Assessment of phage stability under various environmental conditions, including Cu2+ exposure.
Main Results:
- Phage vB_Mm5 exhibits a 10-minute latent period and a burst size of 30 (±5).
- The phage demonstrates high tolerance to elevated temperatures and Cu2+.
- Its genome (163,232 bp dsDNA) contains 229 open reading frames, including genes for enhanced predation and host defense evasion.
Conclusions:
- vB_Mm5 is genetically distinct from other sequenced M. morganii phages.
- The phage lacks known virulence genes.
- vB_Mm5 shows potential as a therapeutic agent against M. morganii infections.
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