Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Stability01:53

RNA Stability

33.1K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.1K
What is Gene Expression?01:36

What is Gene Expression?

8.3K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
8.3K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

849
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
849
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

22.0K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.0K
Types of RNA01:20

Types of RNA

5.5K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
5.5K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.9K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thermal titration molecular dynamics (TTMD) reveals the temperature-dependent structural stability of major royal jelly protein 1.

Journal of molecular graphics & modelling·2026
Same author

The Impact of <i>pfmdr1</i> Gene Copy Number on the Efficacy of Dihydroartemisinin-Piperaquine in Treating Malaria in West Sumba and Kupang Districts, East Nusa Tenggara, Indonesia.

Journal of parasitology research·2026
Same author

New Updates in Adipocytes and Adipose Tissue: 2nd Edition.

Life (Basel, Switzerland)·2026
Same author

DIA-NN EasyFilter Workflow for the Fast and User-Friendly Critical Assessment and Visualization of DIA-NN Proteomics Analysis Outcome.

Journal of proteome research·2026
Same author

A conserved hormonal signalling-H2A.Z axis rapidly reorganizes 3D chromatin interactions in adipocyte thermogenesis.

Nature metabolism·2026
Same author

Targeting p38α, p38γ, and ERα with bioactive compounds from royal jelly for anti-breast cancer activity: A comprehensive in-silico study using fingerprint analysis, molecular dynamics, MEP, and PCA.

Biochemical and biophysical research communications·2026

Related Experiment Video

Updated: May 14, 2025

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.3K

RNA modifications and their role in gene expression.

I Made Artika1, Rini Arianti2,3, Máté Á Demény4

  • 1Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Indonesia.

Frontiers in Molecular Biosciences
|May 12, 2025
PubMed
Summary

RNA modifications are crucial for gene expression and understanding them is key to normal development and disease. This review covers RNA modification types, methods, mechanisms, and therapeutic potential.

Keywords:
RNARNA editingRNA modificationepitranscriptomicsgene expressiontherapeutic development

More Related Videos

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
07:46

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

Published on: October 8, 2018

6.9K
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

9.6K

Related Experiment Videos

Last Updated: May 14, 2025

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.3K
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
07:46

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

Published on: October 8, 2018

6.9K
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

9.6K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Regulation

Background:

  • Post-transcriptional RNA modifications are vital regulators of gene expression.
  • Understanding these modifications is essential for normal tissue development and disease susceptibility.
  • Multicellular organisms rely on complex cellular mechanisms to control gene expression.

Purpose of the Study:

  • To review the current understanding of RNA modifications.
  • To discuss the methods used to study RNA modifications.
  • To elucidate the role of RNA modifications in gene expression and therapeutic development.

Main Methods:

  • Literature review of RNA modification research.
  • Analysis of molecular mechanisms underlying RNA modification.
  • Discussion of physiological and diseased states influenced by RNA modification.

Main Results:

  • RNA modifications encompass a diverse range including N6-methyladenosine (m⁶A), inosine (I), and 5-methylcytosine (m⁵C).
  • Various methods exist for studying RNA modifications and their impact on gene expression.
  • RNA modifications play significant roles in both normal biological processes and disease pathogenesis.

Conclusions:

  • RNA modifications are critical regulators of gene expression with implications for health and disease.
  • Further research into RNA modifications offers potential for novel therapeutic strategies.
  • This review provides a comprehensive overview of the field, highlighting current knowledge and future directions.