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Published on: November 7, 2018
Natural Deep Eutectic Solvents for the Extraction of Mulberroside A from White Mulberry Root Bark
Zhiyang Chen1, Zhengyou He1, Jialan Qin1
1School of Pharmacy, Chengdu University, Shiling Town, Chengdu City, Sichuan Province 610106, P.R. China.
Abstract:
In this study, an ultrasonic extraction method of mulberroside A from Morus alba L. based on natural deep eutectic solvents (NADESs) was developed. A total of 24 natural deep eutectic solvents (NADESs) with different formulations were designed to extract M. alba L. powder, and high-performance liquid chromatography (HPLC) was used as the detection method, with the content of mulberroside A as the only evaluation index. The results showed that malic acid:glucose (1:3) contained the highest mulberroside A content of 0.06% with excellent extraction specificity. In addition, the parameters such as water content, extraction temperature, extraction time, and feed/liquid ratio, which have a great influence on NADESs, were optimized, and the antioxidant activity of NADES extracts was tested. Finally, further fine separation of mulberroside A by semidynamic adsorption on porous polymer beads yielded a refined product of mulberroside A under NADES extraction conditions with a purity of 82.9%.

