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Patchouli Ethanol Extract Pogostemon cablin Benth. Against Aging Profiles in Doxorubicin-Induced 3T3-L1 Fibroblast
Teguh Santoso1, Astrid Feinisa Khairani1,2, Muhammad Hasan Bashari1,2,3
1Graduate School of Master Program in Anti-Aging and Aesthetic Medicine Faculty of Medicine, Universitas Padjadjaran Bandung West Java Indonesia.
Objectives:
This research aimed to investigate the potential of Pogostemon cablin Benth's ethanolic extract (PCEE) as an antiaging agent.
Methods:
We treated 3T3-L1 cells induced by doxorubicin with PCEE and assessed them with antiaging markers such as GLB-1 gene and telomere length with polymerase chain reaction (PCR), or reactive oxygen species (ROS) accumulation with 2,7-dichlorofluorescein diacetate (DCFHDA).
Results:
This study demonstrates that PCEE can inhibit ROS accumulation, decrease the expression level of the senescence-associated β-galactosidase (SA-β-gal) gene, GLB-1, and maintain telomere length in doxorubicin-induced 3T3-L1 cells.
Conclusion:
These studies reveal that PCEE is a promising antiaging agent. An in vivo approach should be conducted for further research.
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