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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

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Related Experiment Video

Updated: Jun 26, 2026

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts
09:07

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts

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Decoding Aging through iPSC Reprogramming: Advances and Challenges.

Rui-Lin Li1, Yun-Zeng Zou1, Sheng Kang2

  • 1Department of Cardiovascular Medicine, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.

Aging and Disease
|May 12, 2025
PubMed
Summary
This summary is machine-generated.

Induced pluripotent stem cell (iPSC) technology and CRISPR tools reverse aging hallmarks like senescence and mitochondrial dysfunction. These advancements offer potential for rejuvenation therapies and treating age-related diseases.

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Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research
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Area of Science:

  • Stem Cell Biology
  • Aging Research
  • Gene Editing

Background:

  • Aging is marked by cellular senescence and heightened susceptibility to age-related diseases.
  • Induced pluripotent stem cell (iPSC) technology offers a pathway to reverse aging indicators.
  • Key aging hallmarks include telomere attrition, mitochondrial dysfunction, and oxidative stress.

Purpose of the Study:

  • To explore the potential of iPSC technology and CRISPR tools in reversing aging processes.
  • To investigate methods for mitigating risks associated with iPSC reprogramming.
  • To examine the application of these technologies in disease modeling and therapeutic development.

Main Methods:

  • Utilizing reprogramming factors (Oct4, Sox2, Klf4, c-Myc) for somatic cell reprogramming.
  • Employing partial reprogramming via transient factor expression to rejuvenate cells.
  • Applying CRISPR-based tools for precise epigenetic editing to remove somatic cell signatures.

Main Results:

  • Partial reprogramming resets epigenetic clocks, reduces senescence-associated secretory phenotypes (SASPs), and improves mitochondrial function.
  • Lifespan extension observed in progeroid mouse models following partial reprogramming.
  • Development of non-integrative delivery systems and suicide genes to address tumorigenicity risks.

Conclusions:

  • iPSC and CRISPR technologies represent a transformative approach to delaying aging and restoring cellular vitality.
  • These technologies hold promise for developing novel rejuvenation therapies for age-related disorders.
  • Further research is needed to optimize reprogramming efficiency, ensure safety, and refine epigenetic editing techniques.