Comprehensive wound healing using ETN@Fe₇S₈ complex by positively regulating multiple programmed phases.

Area of Science:

• Biomaterials Science
• Nanotechnology
• Wound Healing Research

Background:

• Effective wound healing necessitates addressing multiple physiological stages, including hemostasis, infection control, inflammation resolution, proliferation, and remodeling.
• Existing nanomaterials often target only specific aspects of wound healing, limiting their overall efficacy.
• A comprehensive approach is needed to address the multifaceted nature of wound repair.

Purpose of the Study:

• To develop a multifunctional nanocomplex for integrated wound treatment.
• To investigate the therapeutic potential of nano-iron sulfide integrated with erythrocyte-templated nanozyme (ETN@Fe7S8).
• To evaluate the capacity of ETN@Fe7S8 to manage various stages of wound healing.

Main Methods:

• Synthesis of Fe7S8 nanocomposite using ETN as a template via solvothermal reaction.
• Assessment of bactericidal activity against methicillin-resistant Staphylococcus aureus (MRSA).
• Evaluation of anti-inflammatory, pro-angiogenic, and cell proliferation effects in vitro and in vivo wound models.

Main Results:

• ETN@Fe7S8 exhibited potent bactericidal effects against MRSA via ferroptosis-like cell death.
• The complex demonstrated significant anti-inflammatory action by inhibiting the NF-κB pathway.
• ETN@Fe7S8 promoted angiogenesis through VEGFA and PI3K/AKT pathway activation, enhanced keratinocyte proliferation, and showed hemostatic properties.

Conclusions:

• ETN@Fe7S8 provides a multifunctional solution for comprehensive wound management.
• The developed nanocomplex effectively addresses infection, inflammation, and promotes tissue regeneration across all healing stages.
• This study presents a promising candidate for advanced wound care therapies.