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Updated: May 3, 2026

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
Published on: May 4, 2018
Exploration of the structural features and anti-oxidative activity of whey protein hydrolysates produced by
Cuicui Duan1, Shunan Hu1, Cheng Wu1
1Key Laboratory of Agro-products Processing Technology, Jilin Provincial Department of Education, Changchun University, 6543 Weixing Road, Changchun, 130022, Jilin province, PR China.
Abstract:
The structural features and antioxidant activities of whey protein hydrolysates (WPHs) were investigated in this study. The secondary structure, fluorescence spectra and molecular weight distribution of WPHs changed with different hydrolysis time and strains. Tricine-SDS-PAGE results presented that some large proteins were hydrolyzed into small peptides (≤15kD). Compared with natural whey protein, the contents of free amino acids (including Tyr, Lys, etc.) in WPHs were increased. In addition, WPHs were not only with excellent scavenging ability to DPPH and hydroxyl radicals, but also could enhance the cell viability, decline ROS level and restore MMP of RAW264.7 cells induced by LPS. Three peptides were synthesized according to the identified results of BIOPEP-UWM database and exhibited high antioxidant activity in vitro and LPS-induced RAW264.7 cells. This study identifies the structural features of WPHs and explore their antioxidant activities and these results will have crucial theoretical significance for commercial development of WPHs.
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