A metabolome-driven deep exploration into Moringa's medicinal efficacy.

Area of Science:

• Pharmacology and Phytochemistry
• Metabolomics
• Herbal Medicine Research

Background:

• Moringa oleifera (Moringa) possesses potential clinical applications, but its precise use remains uncertain.
• Existing research highlights Moringa's therapeutic potential, necessitating advanced methods for accurate exploration.
• Herbal medicines derived from Moringa are widely accepted, driving the need for scientific validation.

Purpose of the Study:

• To develop and validate a novel integrated strategy for the precise exploration of Moringa using a signature metabolome.
• To verify the predicted clinical applications of Moringa in relevant disease models.
• To identify and validate metabonomics-based biomarkers for early disease detection.

Main Methods:

• Application of a novel integrated strategy based on a signature metabolome for Moringa exploration.
• Verification of predicted clinical applications in rat models and disease models.
• Quantitative verification and identification of the FA 18:3/FA 20:2 biomarker for alcoholic fatty liver disease detection.

Main Results:

• A precise exploration strategy for Moringa was successfully demonstrated for the first time.
• The signature metabolic profile accurately predicted clinical applications, verified in disease models.
• The biomarker FA 18:3/FA 20:2 was validated for the earlier detection of alcoholic fatty liver disease.

Conclusions:

• The study presents a convenient and accurate strategy for exploring Moringa oleifera.
• Metabonomics-based biomarkers are effective for the early detection of diseases like alcoholic fatty liver disease.
• This approach validates the use of metabonomics for discovering new indicators in herbal medicine research.