Metabolomic analysis of flavonoid diversity and biosynthetic pathways in whole grains

Whole grains represent key components of a healthy diet, helping to meet the nutritional needs of consumers and playing a crucial role in preventing chronic diseases. Whole grains are rich in various types of flavonoids with antioxidants and health-promoting properties at varying levels. This article defines and elucidates different whole grain types, analyses the advantages and disadvantages of commonly used metabolomics instruments, and systematically organises and classifies flavonoids detected in whole grains. Additionally, we mapped flavonoid biosynthetic pathways and discussed the usefulness of metabolomic techniques in elucidating the functions of key genes involved in flavonoid biosynthesis. The MYB-bHLH-WD40 (MBW) complex regulates flavonoid biosynthesis during seed development, regulating seed colour and flavonoid content. In addition, MBW complex expression is highly tissue-specific; it is preferentially expressed in purple or black tissues. This review describes flavonoid diversity and biosynthetic pathways in whole grains and provides a theoretical foundation for functional whole grain development and usage.