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Published on: July 10, 2014
Enhancing caries-affected dentin bonding with a mussel-inspired primer.
Yuntong Hu1,2,3, Yi He1,2, Dingjie Wang1,2
1Department of Oral Surgery, 920th Hospital of Joint Logistics Support Force, PLA, Teaching Hospital of Kunming Medical University, Kunming, China.
A new mussel-inspired primer, catechol-Lys-methacrylate (CLM), significantly enhances resin bonding to caries-affected dentin (CAD). This biomimetic approach improves dentin bonding strength and stability, offering a promising clinical solution for dental caries.
Area of Science:
- Biomaterials Science
- Dental Materials
- Polymer Chemistry
Background:
- Dental caries leads to significant mineral loss and collagen degradation in dentin, compromising resin-dentin bond strength and durability.
- Current clinical strategies lack effectiveness in improving bonding to caries-affected dentin (CAD).
- Marine mussel adhesion mechanisms provide inspiration for developing advanced biomaterials.
Purpose of the Study:
- To evaluate a mussel-inspired polymerizable monomer, catechol-Lys-methacrylate (CLM), as a primer for enhancing resin bonding to CAD.
- To investigate the interactions between CLM and collagen in CAD.
- To assess the impact of CLM on bond strength, interface stability, and antibacterial properties.
Main Methods:
- Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) analyzed CLM-collagen interactions.
- Microtensile bond strength and nanoleakage tests assessed bonding performance.
- In-situ zymography and SDS-PAGE evaluated interface stability.
- Antibacterial properties were determined using colony-forming units, live/dead staining, and bacterial morphology observation.
Main Results:
- CLM successfully grafted onto CAD collagen via catechol groups, enabling chemical bonding with resin.
- CLM increased immediate CAD bond strength by approximately 30% and reduced nanoleakage by approximately 24%, with sustained effectiveness after aging.
- CLM promoted collagen crosslinking, inhibited enzymatic activity, and exhibited antibacterial properties, enhancing interface stability.
Conclusions:
- The mussel-inspired CLM monomer effectively improves resin bonding to caries-affected dentin.
- CLM acts as a molecular bridge, enhancing both immediate and aged bonding performance by improving collagen stability and conferring antibacterial properties.
- CLM demonstrates significant potential for clinical application in restorative dentistry.
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