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Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
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Intracellular Delivery Enabled by Squeezing Mechanoporation.

Guorui Zhang1, Rong Mu1, Yanfei Ma1,2

  • 1State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China.

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|May 13, 2025
PubMed
Summary
This summary is machine-generated.

Squeeze mechanoporation offers efficient intracellular delivery by creating temporary cell membrane pores. This technique advances cell therapy, gene editing, and vaccine production.

Keywords:
cell deformationintracellular deliverysqueeze mechanoporationviscoelasticity

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Area of Science:

  • Biotechnology
  • Cellular Biology
  • Drug Delivery

Background:

  • Squeeze mechanoporation is an emerging technique for intracellular delivery.
  • It utilizes the viscoelastic properties of cells to create transient micropores.
  • These pores facilitate the delivery of various exogenous substances into cells.

Purpose of the Study:

  • To comprehensively review current advances in mechanical squeezing-mediated intracellular delivery.
  • To explore the fundamental principles, advantages, applications, and challenges of this technique.
  • To highlight its potential in biomanufacturing and cell therapy.

Main Methods:

  • Review of current literature on squeeze mechanoporation.
  • Analysis of the underlying principles of cell deformation and pore formation.
  • Examination of applications in cell therapy, gene editing, and vaccine production.

Main Results:

  • Squeeze mechanoporation enables efficient, high-throughput delivery of diverse substances like nucleic acids, antibodies, and drugs.
  • The technique offers significant advantages over traditional drug delivery methods.
  • It presents promising solutions for challenges in intracellular delivery.

Conclusions:

  • Squeeze mechanoporation holds immense potential for revolutionizing drug delivery and treatment paradigms.
  • Further optimization and research can enhance its application in biomanufacturing and cell therapy.
  • This method is poised to be a key technology in advancing cellular therapies.