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Functionalized Interventional System for in Situ Hematologic Cell Capture.
Ying Yu1, Yuan Cheng2, Shiwei Xu1
1College of Engineering and Applied Sciences, MOE Key Laboratory of High-Performance Polymer Materials and Technology, Nanjing University, Nanjing 210023, China.
ACS Applied Materials & Interfaces
|May 13, 2025
Summary
A novel in situ hematologic cell capture (FISHC) system uses a functionalized wire to directly isolate fetal nucleated red blood cells (FNRBCs) from maternal blood. This minimally invasive technology advances noninvasive prenatal diagnosis (NIPD) and liquid biopsy applications.
Area of Science:
- Biomedical Engineering
- Genetics
- Obstetrics
Background:
- Noninvasive prenatal diagnosis (NIPD) using fetal cells is safer than invasive methods but hindered by low fetal nucleated red blood cell (FNRBC) counts and inefficient isolation.
- Current techniques require blood withdrawal and ex vivo processing, limiting practical application.
Purpose of the Study:
- To develop and validate a functionalized interventional system for in situ hematologic cell capture (FISHC) directly from peripheral blood.
- To enable efficient and minimally invasive retrieval of fetal cells for NIPD.
Main Methods:
- A carboxybetaine methacrylate (CBMA)-functionalized medical wire was developed with immobilized FNRBC-specific antibodies.
- The FISHC system was designed for in situ cell capture directly from peripheral blood under physiological conditions.
- In vivo validation was performed in a pregnant monkey model.
Main Results:
- The FISHC system demonstrated high-specificity capture of FNRBCs.
- Feasibility of efficient and minimally invasive fetal cell retrieval was confirmed in a pregnant monkey model.
- The system eliminates the need for blood withdrawal and ex vivo processing.
Conclusions:
- FISHC offers a paradigm shift in cell-based NIPD by enabling direct, in situ fetal cell capture.
- This technology holds significant promise for improving NIPD and expanding to other applications like circulating tumor cell detection and real-time liquid biopsy.
