Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cytoskeletal Proteins in Bacteria01:29

Cytoskeletal Proteins in Bacteria

3.2K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.2K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.4K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.4K
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

5.0K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.0K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

2.2K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.2K
Formation of Higher-order Actin Filaments01:11

Formation of Higher-order Actin Filaments

2.9K
The polymerization of G-actin monomers into filamentous F-actin is a multi-step process. Once the F-actins are formed, they can bundle together in different arrangements to form higher-order networks and regulate cellular functions. Common examples include the formation of lamellipodia and filopodia at the cell's leading edge by actin reorganization in a migrating cell. The microvilli on the brush border epithelial cells are also formed through the F-actin network.
The high-order actin...
2.9K
Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

1.9K
Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intestinal metabolic characteristics of Smilax china L. pectic polysaccharide and prediction of its gut microbiota-mediated mechanism.

International journal of biological macromolecules·2026
Same author

Chronic Low-Dose Cadmium Exposure Impairs Adult Hippocampal Neurogenesis by Suppressing Noncanonical Wnt5a Signaling.

Journal of applied toxicology : JAT·2026
Same author

Mutation of a conserved lysine in the RdRp fingers domain broadly attenuates orthobunyaviruses.

Communications biology·2026
Same author

Bionic phase-transitioned α-lactalbumin coating for efficient separation of structurally similar environmental endocrine disruptors by open tubular capillary electrochromatography.

Journal of chromatography. A·2026
Same author

Mosquito- and biting-midge-borne arboviruses in Western Yunnan's border region, China.

Parasites & vectors·2026
Same author

Integrating Adverse Outcome Pathway-Bayesian Network and Physiologically Based Pharmacokinetic Modeling for Quantitative Prediction of Developmental Neurotoxicity of F-53B under Human-Relevant Exposure Scenarios.

Environmental science & technology·2026

Related Experiment Video

Updated: May 14, 2025

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
12:58

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Published on: May 25, 2017

8.9K

Lydicamycins induce morphological differentiation in actinobacterial interactions.

Scott A Jarmusch1, Morten D Schostag1, Zhijie Yang1,2

  • 1Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.

Applied and Environmental Microbiology
|May 13, 2025
PubMed
Summary

Lydicamycins produced by Streptomyces trigger developmental changes in other actinobacteria. This discovery reveals a new role for these compounds in microbial interactions and ecological signaling within soil microbiomes.

Keywords:
Streptomycesactinobacteriamass spectrometry imagingmicrobial interactionsmulti-omics

More Related Videos

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
06:08

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

Published on: January 25, 2019

12.8K
Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus
11:45

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus

Published on: June 20, 2018

9.4K

Related Experiment Videos

Last Updated: May 14, 2025

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
12:58

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Published on: May 25, 2017

8.9K
Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
06:08

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

Published on: January 25, 2019

12.8K
Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus
11:45

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus

Published on: June 20, 2018

9.4K

Area of Science:

  • Microbiology
  • Chemical Ecology
  • Genomics

Background:

  • Streptomyces are key soil microbes, but their interactions with other actinobacteria are poorly understood.
  • Complex actinobacterial development necessitates multi-omics approaches to study inter-species interactions.
  • This study investigates induced morphogenesis between Kitasatospora sp. P9-2B1 and Streptomyces sp. P9-2B2.

Purpose of the Study:

  • To explore the chemical ecology of secondary metabolites beyond antibiotic discovery.
  • To analyze microbial interactions in dual cultures using multi-omics for robust conclusions.
  • To elucidate the role of lydicamycins in inter-actinobacterial communication and morphogenesis.

Main Methods:

  • Co-cultivation of environmental actinobacterial isolates on potato dextrose agar.
  • Mass spectrometry imaging to identify accumulated metabolites.
  • CRISPR base editing to generate lydicamycin-deficient mutants.
  • Agar diffusion assays to test lydicamycin's effects.
  • Transcriptomics analysis to assess gene expression changes.

Main Results:

  • Streptomyces sp. P9-2B2 induced wave-like sporulation in Kitasatospora sp. P9-2B1.
  • Lydicamycins were identified in the induced sporulation zones.
  • Lydicamycin-deficient mutants abolished the inducible sporulation, confirming lydicamycin's role.
  • Lydicamycin induced sporulation when added later, but was inhibitory if added concurrently.
  • Similar phenomena observed in other isolates and Streptomyces coelicolor strains.
  • Transcriptomics revealed differential gene expression in developmental and stress response pathways.

Conclusions:

  • Lydicamycins mediate previously unrecognized interactions between actinobacteria.
  • Bioactive metabolites play a broader ecological role in soil microbiomes.
  • This study provides a roadmap for future microbial chemical ecology research using mass spectrometry imaging.